干细胞与组织工程实验室
四川大学华西第二医院
实验室负责人：李沁桐

实验室聚焦妇幼疾病的临床难点，对胚胎发育障碍及卵巢癌等妇幼重大疾病的机理及干预措施进行探索性研究。

实验室主要研究方向：
（1）类器官模拟人类早期胚胎发育；
（2）发育障碍致病基因的鉴定与功能研究；
（3）肿瘤放化疗耐药机制研究；
（4）肿瘤免疫治疗新策略基础与临床研究；
（5）细胞治疗基础与临床研究。

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Laboratory of Stem Cell and Tissue Engineering
West China Second University Hospital, Sichuan University
Principal investigator: Qintong Li

Our lab is hosted by Western China Second University Hospital of Sichuan University, a top-ranked Women’s and Children’s Hospital in China.

We are interested in understanding the etiologies of human developmental disorders, and exploring novel approaches to improve cancer treatment. We formulate hypothesis based on multi-omics datasets of patient specimens, and carry out mechanistic studies to identify novel risk genes and dysregulated pathways underlying tumor progression and developmental disorders. Past efforts have led us to identify novel regulators of stem cell self-renewal and genome stability. Ultimately, we hope that these studies will offer clues for fundamental yet challenging questions such as how our brains work. In addition, we collaborate with clinicians and industry partners to develop novel cell therapies to treat cancer and developmental disorders.

Our current research projects focus on:

(1) Using organoid models to understand mechanisms of early human embryogenesis;

(2) Understanding functions of human neurodevelopment disorder risk genes;

(3) Dissecting clinically relevant mechanisms of cancer drug resistance;

(4) Exploring new strategies of immunotherapy;

(5) Optimizing parameters of manufacturing cell therapeutics.

李沁桐, 教授 Qintong Li,Professor	邓璐, 实验室管理员 Deng Lu, Lab Manager denglu@163.com
柏淑君, 副教授 Bai Shujun, Associate Professor baishujun7776@163.com	张佩璇, 博士后 Zhang Peixuan, Postdoc 436069773@qq.com
方银, 助理研究员 Fang Yin, Junior research scientist myt_kkxx2008@126.com	邱童, 助理研究员 Qiu Tong, Junior research scientist qiutong08@hotmail.com
杨超, 硕士研究生 Yang Chao, M.S. candidate yangchao2@stu.scu.edu.cn	贾明珠, 博士研究生 Jia Mingzhu, PhD candidate 1182338322@qq.com
罗宏, 硕士研究生 Luo hong, M.S. candidate luohong@stu.scu.edu.cn	王敏, 硕士研究生 Wang Min, M.S. candidate 2829599565@qq.com
张钰菲, 博士研究生 Zhange Yufei, PhD candidate zhangyufei@stu.scu.edu.cn	陈静红, 博士研究生 Chen jinghong, PhD candidate chenjinghong@stu.scu.edu.cn
谢海童, 博士研究生 Xie haitong, PhD candidate xiehaitong@stu.scu.edu.cn

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Lab Alumni

1. 吴丽梅, Wu Limei, visiting graduate student
2. 王文翔, Wang Wenxiang, visiting graduate student
3. 杨若宁, Yang Ruoning, visiting graduate student
4. 杨颖慧, Yang Yinghui, visiting graduate student
5. 李萍, Li Ping, visiting graduate student
6. 戴谦, Dai Qian, Postdoc
7. 王萍, Wang Ping, visiting graduate student
8. 王荣, Wang Rong, visiting graduate student
9. 王琰, Wang Yan, graduate student
10. 刘小林, Liu Xiaolin, graduate student
11. 罗光平, Luo Guangping, graduate student
12. 张旭东, Zhang Xudong, graduate student
13. 孙勇康, Sun Yongkang, graduate student
14. 雷霆钧, Lei Tingjun, graduate student
15. 栾广信, Luan Guangxin, graduate student
16. 贺寒冰, He Hanbing, graduate student
17. 向小聪, Xiang Xiaocong, graduate student
18. 邓丽, Deng Li, graduate student
19. 连莹莹, Lian Yingying, graduate student
20. 黄迎彬, Huang Yingbin, graduate student
21. 任浙丹, Ren Zhedan, graduate student
22. 赵长宏, Zhao Changhong, graduate student
23. 杨洁, Yang Jie, graduate student
24. 陈斌, Chen Bin, graduate student
25. 于坤雷, Yu KunLei, graduate student
26. 田平平, Tian Pingping, graduate student
1. Extra-cerebral recombination activity of Emx1-Cre and nestin-Cre in the kidney.Wang M, Liu X, Fang Y, Li Q. Front Cell Dev Biol.2024 Oct 16:12:1480217
2. A new subtype of Lynch syndrome associated with MSH2 c.354T>A (p. Y118*) identified in a Chinese family: case report and literature review. Zhong L, Wang W, Duan Y, Song L, Li Z, Yang K, Li Q, Yin R. Front Genet.2024 Oct 7:15:1440179
3. Macrophage manufacturing and engineering with 5'-Cap1 and N1-methylpseudouridine-modified mRNA.Zhang P, Wang Y, Jiang J, Yang C, Liu X, Lei T, Meng X, Yang J, Ding P, Chen J, Li Q. Mol Ther Methods Clin Dev. 2024 Jul 31;32(3):101307
4. PARP inhibitor maintenance treatment for newly diagnosed ovarian cancer patients: a real-world study from China .Chen J, Zhang M, Li K, Duan Y, Zeng J, Li Q, Wang D, Song L, Li Q, Yin R. Front Oncol .2024 Feb 2:14:1336616.
5. A critical time window for leukapheresis product transportation to manufacture clinical-grade dendritic cells with optimal anti-tumor activities. Wang W, Jiang J, Yang C, Meng X, Gao L, Yuan Y, Lei T, Ding P, Yin R, Li Q. Cytotherapy. 2024 Feb;26(2):210-220
6. A real-world study of PARP inhibitors in 75 patients with platinum-sensitive recurrent ovarian cancer from China.Chen J, Zhang M, Li K, Duan Y, Lin X, Zhong L, Li Q, Yin R . Front Oncol. 2024 Jan 8;13:1300199.
7. The nexus of dynamic T cell states and immune checkpoint blockade therapy in the periphery and tumor microenvironment.Luo H, Wang W, Mai J, Yin R, Cai X, Li Q . Front Immunol. 2023 Oct 10;14:1267918
8. A nomogram for predicting breast cancer specific survival in elderly patients with breast cancer: a SEER population-based analysis.Yang R, Wu Y, Qi Y, Liu W, Huang Y, Zhao X, Chen R, He T, Zhong X, Li Q, Zhou L, Chen J . BMC Geriatr. 2023 Sep 25;23(1):594
9. Therapeutic strategies targeting folate receptor α for ovarian cancer.Mai J, Wu L, Yang L, Sun T, Liu X, Yin R, Jiang Y, Li J, Li Q. Front Immunol. 2023 Aug 30;14:1254532
10. Antiandrogen treatment induces stromal cell reprogramming to promote castration resistance in prostate cancer.Wang H, Li N, Liu Q, Guo J, Pan Q, Cheng B, Xu J, Dong B, Yang G, Yang B, Wang X, Gu Y, Zhang G, Lian Y, Zhang W, Zhang M, Li T, Zang Y, Tan M, Li Q, Wang X, Yu Z, Jiang J, Huang H, Qin . Cancer Cell.2023 Jul 10;41(7):1345-1362.e9
11. Single-incision endoscope-assisted breast-conserving surgery and sentinel lymph node biopsy: prospective SINA-BCS cohort study. Lu S, Yang J, Wei T, Li Q, Wu Y, Wang Z, Li H, Wang J, Wang X, Lv Q, Chen J. The British Journal of Surgery. 2023 Mar 22:znad059.
12. Development of a personalized dendritic cell vaccine and single-cell RNA sequencing-guided assessment of its cell type composition. Li Q, Yang C, Tian H, Jiang J, Li P, Zhu X, Lei T, Yin R, Ding P, Bai P, Li Q. Cytotherapy. 2023 Feb;25(2):210-219.
13. Loss-of-function CFTR p.G970D missense mutation might cause congenital bilateral absence of the vas deferens and be associated with impaired spermatogenesis. Hou JW, Li XL, Wang L, Dai CL, Li N, Jiang XH, Tan YQ, Tian EP, Li QT, Xu WM. Asian Journal of Andrology. 2023 Jan-Feb;25(1):58-65.
14. Diagnostic value of core needle biopsy for determining HER2 status in breast cancer, especially in the HER2-low population. Chen R, Qi Y, Huang Y, Liu W, Yang R, Zhao X, Wu Y, Li Q, Wang Z, Sun X, Wei B, Chen J. Breast Cancer Research and Treatment. 2023 Jan;197(1):189-200.
15. Effect of adjuvant chemotherapy on the survival outcomes of elderly breast cancer: A retrospective cohort study based on SEER database. Wu Y, Qi Y, Yang J, Yang R, Lui W, Huang Y, Zhao X, Chen R, He T, Lu S, Wang Z, Li H, Sun X, Li Q, Zhou L, Chen J. Journal of Evidence-based Medicine. 2022 Dec;15(4):354-364.
16. ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression. Li N, Liu Q, Han Y, Pei S, Cheng B, Xu J, Miao X, Pan Q, Wang H, Guo J, Wang X, Zhang G, Lian Y, Zhang W, Zang Y, Tan M, Li Q, Wang X, Xiao Y, Hu G, Jiang J, Huang H, Qin J. Nature Communications. 2022 Nov 26;13(1):7281.
17. Exploration of the regulatory relationship between KRAB-Zfp clusters and their target transposable elements via a gene editing strategy at the cluster specific linker-associated sequences by CRISPR-Cas9. Zhang Y, He F, Zhang Y, Dai Q, Li Q, Nan J, Miao R, Cheng B. Mobile DNA. 2022 Nov 10;13(1):25.
18. COVID-19 Vaccination Status and Hesitancy among Breast Cancer Patients after Two Years of Pandemic: A Cross-Sectional Survey. Liu W, Wu Y, Yang R, Chen R, Huang Y, Zhao X, Xie M, Li Q, Wang Q, Chen J. Vaccines (Basel). 2022 Sep 15;10(9):1530.
19. Dynamic regulation of P-TEFb by 7SK snRNP is integral to the DNA damage response to regulate chemotherapy sensitivity. Fang Y, Wang Y, Spector BM, Xiao X, Yang C, Li P, Yuan Y, Ding P, Xiao ZX, Zhang P, Qiu T, Zhu X, Price DH, Li Q. iScience. 2022 Aug 4;25(9):104844.
20. Genomic, Metabolic, and Immunological Characterization of GMP-Grade Mycobacterium phlei. Qiu T, Luo G, Jiang J, Ding P, Li Q. Microbiology Spectrum. 2022 Jun 21:e0007022.
21. Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity. Ren J, Li N, Pei S, Lian Y, Li L, Peng Y, Liu Q, Guo J, Wang X, Han Y, Zhang G, Wang H, Li Y, Jiang J, Li Q, Tan M, Peng J, Hu G, Xiao Y, Li X, Lin M, Qin J. Journal of Clinical Investigation. 2022 Apr 15;132(8):e153167.
22. Excipient of paclitaxel induces metabolic dysregulation and unfolded protein response. Dai Q, Liu X, He T, Yang C, Jiang J, Fang Y, Fu Z, Yuan Y, Bai S, Qiu T, Yin R, Ding P, Chen J, Li Q. iScience. 2021 Sep 25;24(10):103170.
23. The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability. Pan Q, Zhong S, Wang H, Wang X, Li N, Li Y, Zhang G, Yuan H, Lian Y, Chen Q, Han Y, Guo J, Liu Q, Qiu T, Jiang J, Li Q, Tan M, Yin H, Peng J, Xiao Y, Qin J. Molecular Cell. 2021 Jul 1; 81(13):2736-2751.e8.
24. SETD2 Restricts Prostate Cancer Metastasis by Integrating EZH2 and AMPK Signaling Pathways. Yuan H, Han Y, Wang X, Li N, Liu Q, Yin Y, Wang H, Pan L, Li L, Song K, Qiu T, Pan Q, Chen Q, Zhang G, Zang Y, Tan M, Zhang J, Li Q, Wang X, Jiang J, Qin J. Cancer Cell. 2020 Sep 14;38(3):350-365.e7.
25. Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations. Yang T, Zhang J, Ke D, Yang W, Tang M, Jiang J, Cheng G, Li J, Cheng W, Wei Y, Li Q, Naismith JH, Zhu X. Nature Communications. 2019 Jul 19;10(1):3214.
26. Chromatin remodeling ATPase BRG1 and PTEN are synthetic lethal in prostate cancer. Ding Y, Li N, Dong B, Guo W, Wei H, Chen Q, Yuan H, Han Y, Chang H, Kan S, Wang X, Pan Q, Wu P, Peng C, Qiu T,J, Hui J, Peng J, Liu Y, Qiu T, Jiang M, Pan Q, Han Y, Wang X, Li Q, Gao D, Xue W, Qin J. Journal of Clinical Investigation. 2018 Nov 29;127(9):3375-3391.
27. Analysis of the genotype-phenotype correlation in patients with phenylketonuria in mainland China. Li N, He C, Li J, Tao J, Liu Z, Zhang C, Yuan Y, Jiang H, Zhu J, Deng Y, Guo Y, Li Q, Yu P, Wang Y. Scientific Reports. 2018 Jul 26;8(1):11251.
28. Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation. Lei T, Zhang P, Zhang X, Xiao X, Zhang J, Qiu T, Dai Q, Zhang Y, Min L, Li Q, Yin R, Ding P, Li N, Qu Y, Mu D, Qin J, Zhu X, Xiao ZX, Li Q. Nature Communications. 2018 May 14;9(1):1876.
29. ATM inhibition induces synthetic lethality and enhances sensitivity of PTEN-deficient breast cancer cells to cisplatin. Li K, Yan H, Guo W, Tang M, Zhao X, Tong A, Peng Y, Li Q, Yuan Z. Experimental Cell Research. 2018 May 1;366(1):24-33.
30. Long non-coding RNA linc00460 promotes epithelial-mesenchymal transition and cell migration in lung cancer cells. Li K, Sun D, Gou Q, Ke X, Gong Y, Zuo Y, Zhou JK, Guo C, Xia Z, Liu L, Li Q, Dai L, Peng Y. Cancer Letters. 2018 Apr 28; 420:80-90.
31. Histone methyltransferase SETD2 modulates alternative splicing to inhibit intestinal tumorigenesis. Yuan H, Li N, Fu D, Ren J, Hui J, Peng J, Liu Y, Qiu T, Jiang M, Pan Q, Han Y, Wang X, Li Q, Qin J. Journal of Clinical Investigation. 2017 Sep 1;127(9):3375-3391.
32. AKT-mediated stabilization of histone methyltransferase WHSC1 promotes prostate cancer metastasis. Li N, Xue W, Yuan H, Dong B, Ding Y, Liu Y, Jiang M, Kan S, Sun T, Ren J, Pan Q, Li X, Zhang P, Hu G, Wang Y, Wang X, Li Q, Qin J. Journal of Clinical Investigation. 2017 Apr 3;127(4):1284-1302.
33. p53 and p73 Regulate Apoptosis but Not Cell-Cycle Progression in Mouse Embryonic Stem Cells upon DNA Damage and Differentiation. He H, Wang C, Dai Q, Li F, Bergholz J, Li Z, Li Q, Xiao ZX. Stem Cell Reports. 2016 Dec 13;7(6):1087-1098 (co-corresponding author).
34. Inhibition of nuclear factor-kappa B enhances the tumor growth of ovarian cancer cell line derived from a low-grade papillary serous carcinoma in p53-independent pathway. Xiao X, Yang G, Bai P, Gui S, Nyuyen TM, Mercado-Uribe I, Yang M, Zou J, Li Q, Xiao J, Chang B, Liu G, Wang H, Liu J. BMC Cancer. 2016 Aug 2;16:582.
35. IGF-1 regulates the SIRT1-p53 pathway in cellular senescence. Tran D, Bergholz J, Zhang H, He H, Wang Y, Zhang Y, Li Q, Kirkland JL, Xiao ZX. Aging Cell, 2014 Aug;13(4):669-78.
36. A distinct expression pattern of cyclin K in mammalian testes suggests a functional role in spermatogenesis. Xiang X, Deng L, Zhang J, Zhang X, Lei T, Luan G, Yang C, Xiao ZX, Li Q. PLOS ONE, 2014 Jul 8;9(7):e101539.
37. Primordial dwarfism gene links Lin28 to safeguard embryonic stem cell from premature differentiation. Dai Q, Luan G, Deng L, Lei T, Kang H, Song X, Zhang Y, Xiao ZX, Li Q. Cell Reports, 2014 May 8;7(3):735-46.
38. PKC phosphorylates HEXIM1 and regulates P-TEFb activity. Fujinaga K, Barboric M, Li Q , Luo Z, Price DH, Peterlin BM. Nucleic Acids Research, 2012 Oct;40(18):9160-70
39. Cyclin K-containing kinase complexes maintain self-renewal in murine embryonic stem cells. Dai Q, Lei T, Zhao C, Zhong J, Tang YZ, Chen B, Yang J, Li C, Wang S, Song X, Li L, Li Q. Journal of Biological Chemistry, 2012 Jul 20;287(30):25344-52
40. Genetic analysis of P-TEFb function via heterologous nucleic acid tethering systems.Li Q , Peterlin BM. Methods, 2009 Aug;48(4):375-80
41. Cyclin T2 is essential for mouse embryogenesis. Kohoutek J, Li Q, Blazek D, Luo Z, Jiang H, Peterlin BM. Molecular and Cellular Biology, 2009 Jun;29(12):3280-5 (co-first author)
42. Nucleophosmin interacts with HEXIM1 and regulates RNA polymerase II transcription.Gurumurthy M, Tan CH, Ng R, Zeiger L, Lau J, Lee J, Dey A, Philp R, Li Q , Lim TM, Price DH, Lane DP, Chao SH. Journal of Molecular Biology, 2008 Apr 25;378(2):302-17
43. MicroRNA regulation of stem cell fate.Li Q , Gregory RI. Cell Stem Cell, 2008 Mar 6;2(3):195-6
44. Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.Jeronimo C, Forget D, Bouchard A,Li Q , Chua G, Poitras C, Thérien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B. Molecular Cell, 2007 Jul 20;27(2):262-74 (cover article)
45. HEXIM1 is a promiscuous double-stranded RNA-binding protein and interacts with RNAs in addition to 7SK in cultured cells. Li Q , Cooper JJ, Altwerger GH, Feldkamp MD, Shea MA, Price DH. Nucleic Acids Research, 2007 35(8):2503-12
46. Analysis of the large inactive P-TEFb complex indicates that it contains one 7SK molecule, a dimer of HEXIM1 or HEXIM2, and two P-TEFb molecules containing Cdk9 phosphorylated at threonine 186.Li Q , Price JP, Byers SA, Cheng D, Peng J, Price DH. Journal of Biological Chemistry, 2005 Aug 5;280(31):28819-26
47. HEXIM2, a HEXIM1-related protein, regulates positive transcription elongation factor b through association with 7SK.Byers SA, Price JP, Cooper JJ, Li Q, Price DH. Journal of Biological Chemistry, 2005 Apr 22;280(16):16360-7
48. Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor.Michels AA, Fraldi A, Li Q, Adamson TE, Bonnet F, Nguyen VT, Sedore SC, Price JP, Price DH, Lania L, Bensaude O. EMBO Journal, 2004 Jul 7;23(13):2608-19 (co-first author)

	如果你热爱生物医学探索性研究，欢迎加入我们。
	We welcome motivated individuals to join our research team.
地址:	四川大学华西第二医院西部妇幼医学研究院，成都市人民南路三段 17号， 610064
电子邮件:	liqintong@scu.edu.cn
Address:	The West China Second University Hospital of Sichuan University No.20, Section 3, Renmin South Road, Chengdu,Sichuan,610041
E-mail:	liqintong@scu.edu.cn


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